p>INEX has established a well equipped 210 m² class 2 molecular biology and cell culture facility. Its staff has wide ranging expertise in physiology, molecular biology, fluidics, and medical device development. Device development is supported by extensive
design modelling and simulation,
microfabrication, packaging,
metrology inspection and test,
microscopy, and
characterisation expertise and facilities. INEX obtained ISO9001:2000 certification in Q1 2007.
Capability
Mammalian Tissue Culture
- Primary and secondary cell lines
- Biocompatibility testing (viability and proliferation)
- Functional testing
Molecular Biology
- Quantitative-PCR
- Micro-array assay development
- Magnetic beads
- Fluorescence
Functional Testing
- Immunocytochemistry
- Fura-2 ratiometric imaging of intracellular calcium
- Ratiometric imaging of cell membrane potential using voltage sensitive dyes
- BCECF monitoring of intracellular pH
Device Development
- Design, modelling and simulation, fabrication (silicon, glass, polymers)
- Micro- and milli-fluidic based sample processing and manipulation
- Separation, concentration, mixing
- Bead and array-based detection
- Fluorescence, particle imaging, SPR, electrochemical
- Stimulation and sensing electrodes
- Topographic, chemical and biological modification
- Chemical and biological functionalisation
- Functional testing
- Biomolecular and cell based
Characterisation
Microscopy
Equipment
Mammalian Tissue Culture
- Class 2 safety cabinets
- Sanyo CO² incubators
Molecular Biology
- Eppendorf PCR Mastercycler
- Stratagene Mx-4000 Q-PCR
- UVitec BTS-20.M UV imaging station
- Varian Cary Eclipse fluorescence spectrophotometer
- Varian Cary 50 UV-Visible spectrophotometer